rabbit polyclonal anti spike s2 antibody  (Sino Biological)

Journal: Vaccines

Article Title: Simian Immunodeficiency Virus-Based Virus-like Particles Are an Efficient Tool to Induce Persistent Anti-SARS-CoV-2 Spike Neutralizing Antibodies and Specific T Cells in Mice

doi: 10.3390/vaccines13030216

Figure Lengend Snippet: Analysis of S-Delta expression in transfected cells. ( A ) 293T Lenti-X cells were transfected with S-Delta-expressing plasmid (pSpike-INC3) and membrane stained with an anti-S1 neutralizing mAb (COVA1-18) or a commercial anti-S2 polyclonal antibody, and anti-human Alexa Fluor 488 or anti-rabbit PE secondary antibodies. The % of Spike-expressing cells is indicated in each plot. Representative results of 3 independent experiments are shown. ( B ) CLSM analysis of 293T Lenti-X cells transfected with S-Delta (panels a , b ), pSIVGag-GFP (panels c , d ) or both (panels e – f ), and membrane stained with anti-S2 polyclonal antibody followed by anti-rabbit Alexa Fluor 594 secondary Ab (shown in red). Gag-GFP expression is shown in green. Blue color represents nuclei stained with DAPI. Scale bars are 5 μm. Two images are shown from one representative of 3 independent experiments.

Article Snippet: Twenty-four hours after transfection, cells were washed and directly stained, prior to fixation, with anti-S2 polyclonal Ab (Cat: 40590-T62, Sino Biological, 1:50) followed by goat anti-rabbit IgG Alexa Fluor 595 (Cat: A-11072, Thermo Fisher Scientific, Waltham, MA, USA; 1:200) secondary Ab to detect membrane expression.

Techniques: Expressing, Transfection, Plasmid Preparation, Membrane, Staining

Journal: Vaccines

Article Title: Simian Immunodeficiency Virus-Based Virus-like Particles Are an Efficient Tool to Induce Persistent Anti-SARS-CoV-2 Spike Neutralizing Antibodies and Specific T Cells in Mice

doi: 10.3390/vaccines13030216

Figure Lengend Snippet: S-Delta protein is incorporated on VLP/S-Delta. ( A ) TEM observation of 293T Lenti-X cells transfected to produce VLP/S-Delta pseudotyped with In.G (left panels) or Co.G (right panels) after membrane staining with anti-S1 COVA1-18 mAb. Two representative images are shown for each sample. Bars: 0.2 μm. Results from one representative of 2 independent experiments are shown. ( B ) CLSM analysis of VLP/S-Delta produced with In.G (left panels) or Co.G (right panels) and stained with polyclonal anti-S2 Ab followed by secondary Ab anti-rabbit Alexa Fluor 594. Panels at the top depict the merged images. Yellow dots indicate the overlapping green (SIVGag-GFP) and red (S-Delta) signals. The panels at the bottom depict the single green or red fluorescent signal. Images from one representative of 2 independent experiments are shown. Bars: 5 μm.

Article Snippet: Twenty-four hours after transfection, cells were washed and directly stained, prior to fixation, with anti-S2 polyclonal Ab (Cat: 40590-T62, Sino Biological, 1:50) followed by goat anti-rabbit IgG Alexa Fluor 595 (Cat: A-11072, Thermo Fisher Scientific, Waltham, MA, USA; 1:200) secondary Ab to detect membrane expression.

Techniques: Transfection, Membrane, Staining, Produced